Metabolism of 2,4-Dichlorophenoxyacetic Acid in 2,4-Dichlorophenoxyacetic Acid-Resistant Soybean Callus Tissue

Metabolism of 2,4-dichlorophenoxyacetic Acid in 2,4-dichlorophenoxyacetic Acid-resistant soybean callus tissue.

Three 2,4-dichlorophenoxyacetic acid (2,4-D) -resistant root callus tissue lines of Glycine max L. Merrill var. Acme were derived by culturing callus tissue 2 to 6 months on 40 milligrams per liter 2,4-D and designated 40R, 40B, and 40C. Tissue line 40R had a lower level of 2,4-D uptake in 2-week-old tissue which disappeared in 3.5-week-old tissue and less free 2,4-D following incubation for 24...

Evidence for compartmentalization of conjugates of 2,4-dichlorophenoxyacetic Acid in soybean callus tissue.

Soybean Glycine max L. Merrill var. Amsoy 71 root callus tissue labeled with [1-(14)C]2,4-dichlorophenoxyacetic acid (2,4-D) which was subsequently incubated for 24 hours in the absence of 2,4-D, released considerable amounts of label into the media. These results led to an examination of the efflux of 2,4-D and 2,4-D metabolites during a 6-hour time period. Fifty% of the free 2,4-D was lost in...

Industrial toxicology; 2,4-dichlorophenoxyacetic acid.

2,4-Dichlorophenoxyacetic Acid-enhanced Phosphorylation of Soybean Nuclear Proteins.

In vitro nuclear protein phosphorylation is enhanced in nuclei isolated from 2,4-dichlorophenoxyacetic acid (2,4-d)-treated mature soybean (Glycine max) hypocotyl relative to nuclei from untreated tissue. Increased nuclear protein phosphorylation correlates with increased levels of nuclear protein kinase activity. These changes generally parallel previously reported 2,4-d-enhanced RNA polymeras...

A comparison of 2,4-dichlorophenoxyacetic Acid metabolism in cultured soybean cells and in embryogenic carrot cells.

The removal or reduction in concentration of auxin is often a successful method for obtaining morphogenesis in cell cultures of higher plants, such as carrot, but not for soybean. For this reason, the metabolism of one auxin, 2,4-dichlorophenoxyacetic acid (2,4-D), was compared in both carrot and soybean cells. Whereas soybean cells conjugated a high percentage of their 2,4-D to amino acids, ca...